Anti-ATP4A Antibody Picoband®

SKU: A08198-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: ATP4A

Specificity:

Background: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4A, which shares 96.2% amino acid (aa) sequence identity with mouse and rat ATP4A.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Maeda, M., Oshiman, K.-I., Tamura, S., Futai, M. Human gastric (H(+) + K(+))-ATPase gene: similarity to (Na(+) + K(+))-ATPase genes in exon/intron organization but difference in control region. J. Biol. Chem. 265: 9027-9032, 1990. 2. Song, I., Yamada, T., Trent, J. M. Mapping of the gene encoding the alpha-subunit of the human H(+),K(+)-ATPase to chromosome 19q13.1 by fluorescent in situ hybridization. Genomics 14: 547-548, 1992.

Uniprot ID: P20648

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 495

Protein Name: Potassium-transporting ATPase alpha chain 1

Gene Full Name: ATPase H+/K+ transporting subunit alpha

Synonyms: ATP4A; Proton pump; ATP6A