Anti-ATP1A4 Antibody Picoband®

SKU: A09005

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATP1A4

Specificity:

Background: Sodium/potassium-transporting ATPase subunit alpha-4 is an enzyme that in humans is encoded by the ATP1A4 gene. The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen: E.coli-derived human ATP1A4 recombinant protein (Position: K45-Q680). Human ATP1A4 shares 84.2% and 84.1% amino acid (aa) sequence identity with mouse and rat ATP1A4, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Buetow, K. H., Nishimura, D., Nakamura, Y., Jiang, O., Murray, J. C. A detailed multipoint gene map of chromosome 1q. Genomics 8: 13-21, 1990. Note: Erratum: Genomics 9: 564 only, 1991. 2. Jimenez, T., McDermott, J. P., Sanchez, G., Blanco, G. Na,K-ATPase alpha-4 isoform is essential for sperm fertility. Proc. Nat. Acad. Sci. 108: 644-649, 2011. 3. Keryanov, S., Gardner, K. L. Physical mapping and characterization of the human Na,K-ATPase isoform, ATP1A4. Gene 292: 151-166, 2002.

Uniprot ID: Q13733

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 480

Protein Name: Sodium/potassium-transporting ATPase subunit alpha-4

Gene Full Name: ATPase Na+/K+ transporting subunit alpha 4

Synonyms: ATP1A4; ATP1AL2; Sodium/potassium-transporting ATPase subunit alpha-4; Na(+)/K(+) ATPase alpha-4 subunit; Sodium pump subunit alpha-4