Anti-Aquaporin 4/AQP4 Antibody Picoband®

SKU: PA1937

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat,Mouse,Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat

Gene Name: AQP4

Specificity: No cross reactivity with other proteins.

Background: AQP4 (Aquaporin 4), also known as MERCURIAL-INSENSITIVE WATER CHANNEL; MIWC, is protein which in humans is encoded by the AQP4 gene. The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. By fluorescence in situ hybridization, Lu et al. (1996) determined that the AQP4 gene maps to 18q11.2-q12.1. By interspecific backcross analysis, Turtzo et al. (1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18. Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al. (1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers. Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related rat and mouse sequences by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Frigeri, A., Nicchia, G. P., Verbavatz, J. M., Valenti, G., Svelto, M. Expression of aquaporin-4 in fast-twitch fibers of mammalian skeletal muscle. J. Clin. Invest. 102: 695-703, 1998. 2. Lu, M., Lee, M. D., Smith, B. L., Jung, J. S., Agre, P., Verdijk, M. A. J., Merkx, G., Rijss, J. P. L., Deen, P. M. T. The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 3. Turtzo, L. C., Lee, M. D., Lu, M., Smith, B. L., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Agre, P. Cloning and chromosomal localization of mouse aquaporin 4: exclusion of a candidate mutant phenotype, ataxia. Genomics 41: 267-270, 1997.

Uniprot ID: P55087

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight: 34830 MW

Gene ID: 361

Protein Name: Aquaporin-4

Gene Full Name: aquaporin 4

Synonyms: AQP 4; AQP4; aquaporin 4; HMIWC2; MIWC; NMO-IgG; WCH4