Anti-Aquaporin 0/MIP Antibody Picoband®

SKU: PA2110

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat

Gene Name: MIP

Specificity: No cross reactivity with other proteins.

Background: Lens fiber major intrinsic protein also called MIP26 or MP26 is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0, identical to the related rat sequence, and different from the related human sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000. 2. Francis, P., Chung, J.-J., Yasui, M., Berry, V., Moore, A., Wyatt, M. K., Wistow, G., Bhattacharya, S. S., Agre, P. Functional impairment of lens aquaporin in two families with dominantly inherited cataracts. Hum. Molec. Genet. 9: 2329-2334, 2000. 3. Pisano MM, Chepelinsky AB (Mar 1992). Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens. Genomics 11 (4): 981–90.

Uniprot ID: P09011

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 27891 MW

Gene ID: 25480

Protein Name: Lens fiber major intrinsic protein

Gene Full Name: major intrinsic protein of lens fiber

Synonyms: AQP 0 antibody; AQP0 antibody; Aquaporin antibody; Aquaporin0 antibody; Lens fiber major intrinsic protein antibody; LIM 1 antibody; LIM1 antibody; Major intrinsic protein 26kD antibody; Major intrinsic protein of lens fiber antibody; MIP 26 antibody; MIP antibody; MIP26 antibody; MP 26 antibody; MP26 antibody; PALM antibody