Anti-APTX Antibody Picoband®

SKU: A02968-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: APTX

Specificity:

Background: This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Immunogen: E.coli-derived human APTX recombinant protein (Position: E46-Q356). Human APTXshares 84.8% and 80.9% amino acid (aa) sequence identity with mouse and rat APTX, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ahel, I., Rass, U., El-Khamisy, S. F., Katyal, S., Clements, P. M., McKinnon, P. J., Caldecott, K. W., West, S. C. The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates. Nature 443: 713-716, 2006. 2. Amouri, R., Moreira, M.-C., Zouari, M., El Euch, G., Barhoumi, C., Kefi, M., Belal, S., Koenig, M., Hentati, F. Aprataxin gene mutations in Tunisian families Neurology 63: 928-929, 2004. 3. Castellotti, B., Mariotti, C., Rimoldi, M., Fancellu, R., Plumari, M., Caimi, S., Uziel, G., Nardocci, N., Moroni, I., Zorzi, G., Pareyson, D., Di Bella, D., Di Donato, S., Taroni, F., Gellera, C. Ataxia with oculomotor apraxia type 1 (AOA1): novel and recurrent aprataxin mutations, coenzyme Q10 analyses, and clinical findings in Italian patients. Neurogenetics 12: 193-201, 2011.

Uniprot ID: Q7Z2E3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 41 kDa

Calculated Molecular Weight: 41 kDa

Gene ID: 54840

Protein Name: Aprataxin

Gene Full Name: aprataxin

Synonyms: Forkhead-associated domain histidine triad-like protein; FHA-HIT; EC:3.6.1.71; AXA1; AXA 1