Anti-Annexin VI Antibody Picoband® (monoclonal, 4C4)

SKU: M03735-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: ANXA6

Specificity:

Background: Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization. The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal. Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion.

Immunogen: E. coli-derived human Annexin VI recombinant protein (Position: N395-L665).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Davies, A. A., Moss, S. E., Crompton, M. R., Jones, T. A., Spurr, N. K., Sheer, D., Kozak, C., Crumpton, M. J.The gene coding for the p68 calcium-binding protein is localized to bands q32-q34 of human chromosome 5, and to mouse chromosome 11.Hum. Genet. 82: 234-238, 1989. 2. Smith, P. D., Davies, A., Crumpton, M. J., Moss, S. E.Structure of the human annexin VI gene.Proc. Nat. Acad. Sci. 91: 2713-2717, 1994. 3. Thomas, D. D. H., Kaspar, K. M., Taft, W. B., Weng, N., Rodenkirch, L. A., Groblewski, G. E.Identification of annexin VI as a Ca(2+)-sensitive CRHSP-28-binding protein in pancreatic acinar cells.J. Biol. Chem. 277: 35496-35502, 2002.

Uniprot ID: P08133

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 4C4

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight:

Gene ID: 309

Protein Name: Annexin A6

Gene Full Name: annexin A6

Synonyms: 67 kDa calelectrin; Annexin 6; annexin A6; Annexin VI; ANX6; ANXA6; Calphobindin II; CBP68; Chromobindin 20; CPB II; Lipocortin VI; p68; p70; Protein III