Anti-ANGPTL4 Antibody Picoband®

SKU: A01147-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ANGPTL4

Specificity:

Background: Angiopoietin-like 4 is a protein that in humans is encoded by the ANGPTL4 gene.[5][6][7] Alternatively spliced transcript variants encoded with different isoforms have been described. This gene was previously referred to as ANGPTL2, HFARP, PGAR, or FIAF but has been renamed ANGPTL4. This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

Immunogen: E.coli-derived human ANGPTL4 recombinant protein (Position: D39-S406). Human ANGPTL4 shares 75.6% and 76.6% amino acid (aa) sequence identity with mouse and rat ANGPTL4, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Backhed, F., Ding, H., Wang, T., Hooper, L. V., Koh, G. Y., Nagy, A., Semenkovich, C. F., Gordon, J. I. The gut microbiota as an environmental factor that regulates fat storage. Proc. Nat. Acad. Sci. 101: 15718-15723, 2004. 2. Dewey, F. E., Gusarova, V., O'Dushlaine, C., Gottesman, O., Trejos, J., Hunt, C., Van Hout, C. V., Habegger, L., Buckler, D., Lai, K.-M. V., Leader, J. B., Murray, M. F., and 14 others. Inactivating variants in ANGPTL4 and risk of coronary artery disease. New Eng. J. Med. 374: 1123-1133, 2016. 3. Galaup, A., Cazes, A., Le Jan, S., Philippe, J., Connault, E., Le Coz, E., Mekid, H., Mir, L. M., Opolon, P., Corvol, P., Monnot, C., Germain, S. Angiopoietin-like 4 prevents metastasis through inhibition of vascular permeability and tumor cell motility and invasiveness. Proc. Nat. Acad. Sci. 103: 18721-18726, 2006.

Uniprot ID: Q9BY76

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40-50 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 51129

Protein Name: Angiopoietin-related protein 4

Gene Full Name: angiopoietin like 4

Synonyms: angiopoietin like 4; Angiopoietin like protein 4; Angiopoietin related protein 4; ANGPTL4; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PSEC0166; UNQ171/PRO197