Anti-ACY-1/ACY1 Antibody Picoband®

SKU: A05650-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ACY1

Specificity:

Background: Aminoacylase-1 is an enzyme that in humans is encoded by the ACY1 gene. This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.

Immunogen: E.coli-derived human ACY-1/ACY1 recombinant protein (Position: E8-A354).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Boldog, F., Erlandsson, R., Klein, G., Sumegi, J. Long-range restriction enzyme maps of DNF15S2, D3S2 and c-raf1 loci on the short arm of human chromosome 3. Cancer Genet. Cytogenet. 42: 295-306, 1989. 2. Cook, R. M., Burke, B. J., Buchhagen, D. L., Minna, J. D., Miller, Y. E. Human aminoacylase-1: cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J. Biol. Chem. 268: 17010-17017, 1993. 3. Ferri, L., Funghini, S., Fioravanti, A., Biondi, E. G., la Marca, G., Guerrini, R., Donati, M. A., Morrone, A. Aminoacylase I deficiency due to ACY1 mRNA exon skipping. Clin. Genet. 86: 367-372, 2014.

Uniprot ID: Q03154

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40-43 kDa

Calculated Molecular Weight: 101272 MW

Gene ID: 95

Protein Name: Aminoacylase-1

Gene Full Name: aminoacylase 1

Synonyms: ACY 1; ACY1; ACY1D; ACYLASE; Aminoacylase 1