Anti-Aconitase 1/ACO1 Antibody Picoband®

SKU: A02781-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Monkey,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: ACO1

Specificity:

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human Aconitase 1/ACO1 recombinant protein (Position: M1-D137).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Azevedo, E. S., Da Silva, M. C. B. O., Lima, A. M. V., Fonseca, E. F., Conseicao, M. M. Human aconitase polymorphism in three samples from northeastern Brazil. Ann. Hum. Genet. 43: 7-10, 1979. 2. Condo, I., Malisan, F., Guccini, I., Serio, D., Rufini, A., Testi, R. Molecular control of the cytosolic aconitase/IRP1 switch by extramitochondrial frataxin. Hum. Molec. Genet. 19: 1221-1229, 2010. 3. Eisenstein, R. S. Iron regulatory proteins and the molecular control of mammalian iron metabolism. Annu. Rev. Nutr. 20: 627-662, 2000.

Uniprot ID: P21399

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 98 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 48

Protein Name: Cytoplasmic aconitate hydratase

Gene Full Name: aconitase 1

Synonyms: ACO1; Aconitase; Aconitase 1; aconitase 1; soluble; ACONS; Citrate hydro lyase; Ferritin repressor protein; IRE BP 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; IRP1