Anti-ACK1/TNK2 Antibody Picoband®

SKU: A02334-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TNK2

Specificity:

Background: Activated CDC42 kinase 1, also known as ACK1, is an enzyme that in humans is encoded by the TNK2 gene. This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is ly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Immunogen: E.coli-derived human TNK2/ACK1 recombinant protein (Position: M491-R1038).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hitomi, Y., Heinzen, E. L., Donatello, S., Dahl, H.-H., Damiano, J. A., McMahon, J. M., Berkovic, S. F., Scheffer, I. E., Legros, B., Rai, M., Weckhuysen, S., Suls, A., De Jonghe, P., Pandolfo, M., Goldstein, D. B., Van Bogaert, P., Depondt, C. Mutations in TNK2 in severe autosomal recessive infantile onset epilepsy. Ann. Neurol. 74: 496-501, 2013. 2. La Torre, A., del Rio, J. A., Soriano, E., Urena, J. M. Expression pattern of ACK1 tyrosine kinase during brain development in the mouse. Gene Expr. Patterns 6: 886-892, 2006. 3. Manser, E., Leung, T., Salihuddin, H., Tan, L., Lim, L. A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363: 364-367, 1993.

Uniprot ID: Q07912

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 115 kDa

Calculated Molecular Weight:

Gene ID: 10188

Protein Name: Activated CDC42 kinase 1

Gene Full Name: tyrosine kinase non receptor 2

Synonyms: ACK; ACK 1; ACK1; Activated CDC42 kinase 1; p21cdc42Hs; TNK2