Anti-14-3-3 sigma/SFN Antibody Picoband®

SKU: PA1797

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: SFN

Specificity: No cross reactivity with other proteins.

Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al. (1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.

Uniprot ID: P31947

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 27774 MW

Gene ID: 2810

Protein Name: 14-3-3 protein sigma

Gene Full Name: stratifin

Synonyms: 14 3 3 protein sigma; 14-3-3; 14-3-3 sigma; HME1; SFN; stratifin; YWHAS