GeneBio Systems

ALD ELISA kit (General species)

Name: ALD ELISA kit (General species)
Brand: GeneBio Systems
SKU: CEA911Ge
Price: 972.00 GBP
Availability: InStock

SKU:CEA911Ge

£972.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 16

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ALD

Target Full Name: Aldosterone

Alternative Names: -

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species ALD / Aldosterone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 24.69-2,000pg/mL

Sensitivity: 9.41pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldosterone (ALD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldosterone (ALD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Aldosterone (ALD). No significant cross-reactivity or interference between Aldosterone (ALD) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to aldosterone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled aldosterone analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of aldosterone in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of aldosterone in the sample.

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;

References Citing This Product: High Salt Intake Down-Regulates Colonic Mineralocorticoid Receptors, Epithelial Sodium Channels and 11β-Hydroxysteroid Dehydrogenase Type 2

Evidence for glucocorticoid&-mediated hypertension after uninephrectomy

Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach

Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder

H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.

Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertension

Effects of renal denervation on monocrotaline induced pulmonary remodeling

Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy

Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy.

Acute adrenal cortex injury during cardiopulmonary bypass in a canine model

ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load