GeneBio Systems

AGE ELISA kit (General species)

Name: AGE ELISA kit (General species)
Brand: GeneBio Systems
SKU: CEB353Ge
Price: 868.00 GBP
Availability: InStock

SKU:CEB353Ge

£868.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 45

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: AGE

Target Full Name: Advanced Glycation End Product

Alternative Names: AGEs; Advanced Glycation End Products

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species AGE / Advanced Glycation End Product

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 98.8-8,000ng/mL

Sensitivity: 38.2ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE). No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Advanced Glycation End Product (AGE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Advanced Glycation End Product (AGE) and unlabeled Advanced Glycation End Product (AGE) (Standards or samples) with the pre-coated antibody specific to Advanced Glycation End Product (AGE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Advanced Glycation End Product (AGE) in the sample.

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;

References Citing This Product: Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM)

A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process

Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D.

Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients.

Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus.

Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications

Nitroxides prevent protein glycoxidation in vitro.

Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea

Ascorbic acid and protein glycation

Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone

Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea