GeneBio Systems

ADPN ELISA kit (Human)

Name: ADPN ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA605Hu
Price: 684.00 GBP
Availability: InStock

SKU:SEA605Hu

£684.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 34

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ADPN

Target Full Name: Adiponectin

Alternative Names: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

Target Species: Human

Uniprot: Q15848

Gene ID: 9370

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human ADPN / Adiponectin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.061ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADPN). No significant cross-reactivity or interference between Adiponectin (ADPN) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin (ADPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology;

References Citing This Product: Relation between afamin level and some inflammatory markers in obese children

Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- …

Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study

Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD?

Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in …

Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity

Relationship between obesity and iron deficiency anemia: is there a role of hepcidin?

Adiponectin may be used as a marker in prediction of diabetic retinopathy patients

Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells

Effect of fermented food consumption on biochemical parameters and adipokines levels

Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension