ACTH ELISA kit (Human)

SKU: CEA836Hu

Size: 96Tests

# of Times Cited in literature: 11

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ACTH

Target Full Name: Adrenocorticotropic Hormone

Alternative Names: Corticotropin; Adrenocorticotropin

Target Species: Human

Uniprot: P17858

Gene ID: 5211

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human ACTH / Adrenocorticotropic Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 12.35-1,000pg/mL

Sensitivity: 5.25pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.

Research Area: Endocrinology;Hormone metabolism;

References Citing This Product: CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release

Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland

Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells

Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue

Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure

Progress in Neuropsychopharmacology & Biological Psychiatry

Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­

Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­

Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients