5-HETE ELISA kit (General species)

SKU: CED739Ge

Size: 96Tests

# of Times Cited in literature: 3

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: 5-HETE

Target Full Name: 5-Hydroxyeicosatetraenoic Acid

Alternative Names: -

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species 5-HETE / 5-Hydroxyeicosatetraenoic Acid

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 9.88-800ng/mL

Sensitivity: 3.87ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxyeicosatetraenoic Acid (5-HETE). No significant cross-reactivity or interference between 5-Hydroxyeicosatetraenoic Acid (5-HETE) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) and unlabeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample.

Research Area: -

References Citing This Product: ACSL4 suppresses glioma cells proliferation via activating ferroptosis

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Ferroptosis is involved in the anti‑tumor effect of lycorine in renal cell carcinoma cells