The development and use of stranded mRNA sequencing have evolved in response to the need for more detailed information about the transcriptome, especially the directionality of transcription. Understanding the orientation of RNA strands is critical for accurate gene expression analysis, identification of antisense transcription, and gaining insights into complex regulatory networks. Stranded mRNA sequencing enables gene expression analysis, alternative splicing analysis, antisense transcription studies, overlapping genes and bidirectional promoters, precise quantification of transcripts, and improved functional genomics.
