Reagents and kits for Protein Assays
Easy Protein Quantitative Kit is a ready-to-use modified Bradford Coomassie-binding, colorimetric method for protein quantification. Under acidic condition, Coomassie Brilliant Blue G-250 binds to proteins providing an immediate shift in absorption maximum from 465 nm to 596 nm and a color change from brown to blue.
The BCA protein assay is one of the most commonly used methods for protein quantification. Under alkaline condition, the reduction of Cu2+ to Cu+ is realized by peptide bonds in proteins (biuret reaction). The amount of reduced copper is directly proportional to the amount of total proteins.