TA-cloning Kits for PCR products
pEASY ®-T1 Cloning Kit is designed for cloning Taq-amplified PCR products. •5 minutes fast ligation of PCR products. •Choice of Kanamycin or Ampicillin resistance genes. •Blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing.
pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released with a single enzyme digestion. •5 minutes fast ligation •Ampicillin resistance gene marker •Blue/white selection •T7, SP6 promoters, M13 forward and M13 reverse primers for...
pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 min. fast ligation •choice of resistance genes •Blue/white selectio •SR primer and M13 forward primer for sequencing
pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed.
5min TA/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and a blunt end factor. The kit provides a highly efficient, 5 minute, one-step cloning strategy at room temperature, for both Taq DNA Pol (3'A-sticky ends) - and Pfu DNA Pol- products (blunt...