VAHTSTM RNA Adapters set3-set6 for Illumina® is intended to use with Illumina NGS RNA-seq library preparation，specifically for multiplexing by indexing primers using indexing adaptors。
The set3 kit (N809) contains 24 different Index adaptors from RNA Adapter 96-01 through RNA Adapter 96-24. The set4 kit (N810) contains 24 different Index adaptors from RNA Adapter 96-25 to RNA Adapter 96-48; The set5 kit (N811) contains 24 different Index connectors from RNA Adapter 96-49 to RNA Adapter 96-72; The set6 kit (N812) contains 24 different Index connectors from the RNA Adapter 96-73 to RNA Adapter 96-96. All adaptors provided in the kit have undergone rigorous quality control and functional verification to ensure the reproducibility and repeatability of the library construction. Storage and expiration date Store at -20 °C. Do not store this product ever in an environment where the temperature is higher than room temperature!
For use with a) VAHTSTM mRNA-seq v2 Library Prep Kit for Illumina (Vazyme #NR601), and b) VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina (Vazyme #NR602). Note: The VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Vazyme #NR603) comes with its own special adapter kit.
16-hour incubation test: 50 μl reaction system containing 5 μl of this product and 1 μg of HindIII-λ DNA, was incubated for 16 hours at 37°C. The electrophoresis analysis showed that the band was not degraded; 50 μl of the reaction system containing 5 μl of this product and 1 μg of T3 DNA was incubated at 37°C for 16 hours. After electrophoresis detection, the band was not degraded.
Redisual Endonuclease: 50 μl of the reaction system was added with 5 μl of this product and 1 μg of φX174 RF I DNA and incubated at 37°C for 4 hours. Using Agarose Electrophoresis detection, the RF II conversion ratio was <10%.
Conjugation concentration assay: A260 absorbance was measured and the difference between the measured and calculated values was <10%. Detection of ligation efficiency: A 35 μl ligation reaction system was added with a 300 bp DNA fragment containing dA overhangs at 1.5 pmol and 2.5 μl of this product, and reacted at 30°C for 10 minutes. Agarose gel electrophoresis showed that the ratio of double-ended linker DNA was >90%.