TP53 (Ab-15) Antibody - Cat. #: CSB-PA063047

SKU: CSB-PA063047

Size :100ul

Clone Number:

Aliases:Antigen NY-CO-13 antibody; BCC7 antibody; Cellular tumor antigen p53 antibody; FLJ92943 antibody; LFS1 antibody; Mutant tumor protein 53 antibody; p53 antibody; p53 tumor suppressor antibody; P53_HUMAN antibody; Phosphoprotein p53 antibody; Tp53 antibody; Transformation related protein 53 antibody; TRP53 antibody; tumor antigen p55 antibody; Tumor protein 53 antibody; Tumor protein p53 antibody; Tumor suppressor p53 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P04637

Immunogen:Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from Human p53.

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Background:

p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:TP53

Research Areas:Epigenetics and Nuclear Signaling;Cancer;Cell biology