GeneBio Systems

T4 ELISA kit (General species)

Name: T4 ELISA kit (General species)
Brand: GeneBio Systems
SKU: CEA452Ge
Price: 774.95 EUR
Availability: InStock

SKU:CEA452Ge

€774,95 EUR

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Quantity

Size: 96Tests

# of Times Cited in literature: 67

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: T4

Target Full Name: Thyroxine

Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species T4 / Thyroxine

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 3.70-300ng/mL

Sensitivity: 1.29ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroxine (T4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroxine (T4). No significant cross-reactivity or interference between Thyroxine (T4) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyroxine (T4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroxine (T4) and unlabeled Thyroxine (T4) (Standards or samples) with the pre-coated antibody specific to Thyroxine (T4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroxine (T4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroxine (T4) in the sample.

Research Area: Endocrinology;Hormone metabolism;

References Citing This Product: Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae

Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis

Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies

Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay.

Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae

Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum

Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice

Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring

Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.

Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus).

Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders