Simian VEGI(Vascular Endothelial Growth Inhibitor) ELISA Kit

SKU: ELK11616

Size:96T

Reactivity:Simian

Alternative Names:TNFSF15; TL1; TL1A; VEGI192A; Tumor Necrosis Factor(ligand)Superfamily,Member 15; TNF Superfamily Ligand TL1A; TNF Ligand-Related Molecule 1

Assay Type:Sandwich

Sensitivity:27.1 pg/mL

Standard:4000 pg/mL

Detection range:62.5-4000 pg/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian VEGI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian VEGI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian VEGI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian VEGI in the samples is then determined by comparing the OD of the samples to the standard curve.

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