Simian Kim1(Kidney Injury Molecule 1) ELISA Kit

SKU: ELK10074

Size:96T

Reactivity:Simian

Alternative Names:HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

Assay Type:Sandwich

Sensitivity:0.079 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:tissue homogenates, urine, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Kidney biomarker;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Kim1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Kim1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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