Simian IL1R1(Interleukin 1 Receptor Type I) ELISA Kit

SKU: ELK11512

Size:96T

Reactivity:Simian

Alternative Names:IL-1R1;CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

Assay Type:Sandwich

Sensitivity:0.048 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;CD & Adhesion molecule;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian IL1R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IL1R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian IL1R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian IL1R1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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