Simian a1AT(Alpha-1-Antitrypsin) ELISA Kit

SKU: ELK11651

Size:96T

Reactivity:Simian

Alternative Names:SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Assay Type:Sandwich

Sensitivity:12.7 ng/mL

Standard:2000 ng/mL

Detection range:31.25-2000 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;Infection immunity;Hepatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian a1AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian a1AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian a1AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian a1AT in the samples is then determined by comparing the OD of the samples to the standard curve.

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