SFTPA1 ELISA kit (Human)

SKU: SEA890Hu

Size: 96Tests

# of Times Cited in literature: 12

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SFTPA1

Target Full Name: Pulmonary Surfactant Associated Protein A1

Alternative Names: PRL; SFTPA; PSAP; PSPA; SFTP; SPA; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; Surfactant Associated Protein A

Target Species: Human

Uniprot: Q8IWL2

Gene ID: 653509

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human SFTPA1 / Pulmonary Surfactant Associated Protein A1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 46.88-3,000pg/mL

Sensitivity: 16.99pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Surfactant Associated Protein A1 (SFTPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Pulmonary Surfactant Associated Protein A1 (SFTPA1). No significant cross-reactivity or interference between Pulmonary Surfactant Associated Protein A1 (SFTPA1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pulmonary Surfactant Associated Protein A1 (SFTPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonary Surfactant Associated Protein A1 (SFTPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pulmonary Surfactant Associated Protein A1 (SFTPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;Pulmonology;

References Citing This Product: Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response

Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps

Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins

The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS

Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge

Expression of surfactant protein-A in exhaled breath condensate of patients with chronic obstructive pulmonary disease

Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)

The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.

Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.

Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid

Evaluation of surfactant proteins A, B, C, and D in articular cartilage, synovial membrane and synovial fluid of healthy as well as patients with osteoarthritis and …

Elevated levels of multifunctional surfactant proteins in cerebrospinal fluid are associated with signs of increased cerebrospinal fluid flow in cranial magnetic …

Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF …

Oxidative Stress and Inflammatory Mediators in Exhaled Breath Condensate of Patients with Pulmonary Tuberculosis. A Pilot Study with a Biomarker Perspective