SDC4 ELISA kit (Human)

SKU: SEB939Hu

Size: 96Tests

# of Times Cited in literature: 5

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SDC4

Target Full Name: Syndecan 4

Alternative Names: SYND4; Amphiglycan; Ryudocan; Ryudocan core protein

Target Species: Human

Uniprot: P31431

Gene ID: 6385

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human SDC4 / Syndecan 4

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.312-20ng/mL

Sensitivity: 0.118ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 4 (SDC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 4 (SDC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Syndecan 4 (SDC4). No significant cross-reactivity or interference between Syndecan 4 (SDC4) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 4 (SDC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 4 (SDC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 4 (SDC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 4 (SDC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Infection immunity;Bone metabolism;Rheumatology;Immunodeficiency;

References Citing This Product: The influence of atorvastatin on parameters of inflammation left ventricular function, hospitalizations and mortality in patients with dilated cardiomyopathy–5-year follow-up

Matrix metalloproteinase 9-mediated shedding of syndecan 4 in response to tumor necrosis factor α: a contributor to endothelial cell glycocalyx dysfunction

Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension

The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension

Aldosterone induces albuminuria via matrix metalloproteinase–dependent damage of the endothelial glycocalyx