RPS6KB1 (Ab-424) Antibody - Cat. #: CSB-PA090473

SKU: CSB-PA090473

Size :100ul

Clone Number:

Aliases:70 kDa ribosomal protein S6 kinase 1 antibody; KS6B1_HUMAN antibody; p70 alpha antibody; P70 beta 1 antibody; p70 ribosomal S6 kinase alpha antibody; p70 ribosomal S6 kinase beta 1 antibody; p70 S6 kinase alpha antibody; P70 S6 Kinase antibody; p70 S6 kinase; alpha 1 antibody; p70 S6 kinase; alpha 2 antibody; p70 S6K antibody; p70 S6K-alpha antibody; p70 S6KA antibody; p70(S6K) alpha antibody; p70(S6K)-alpha antibody; p70-alpha antibody; p70-S6K 1 antibody; p70-S6K antibody; P70S6K antibody; P70S6K1 antibody; p70S6Kb antibody; PS6K antibody; Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody; Ribosomal protein S6 kinase beta 1 antibody; Ribosomal protein S6 kinase beta-1 antibody; Ribosomal protein S6 kinase I antibody; RPS6KB1 antibody; S6K antibody; S6K-beta-1 antibody; S6K1 antibody; Serine/threonine kinase 14 alpha antibody; Serine/threonine-protein kinase 14A antibody; STK14A antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P23443

Immunogen:Peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Background:

RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:RPS6KB1

Research Areas:Epigenetics and Nuclear Signaling;Cell biology;Metabolism;Signal transduction