Recombinant SF3B3 Monoclonal Antibody

SKU: AN301841L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:SF3B3

Target Synonym:SAP;SF3B;KIAA;Splicing factor 3B subunit;Spliceosome-associated protein;Spliceosome-associated protein 130;Splicing factor 3B subunit 3;SF3B3;KIAA0017;SAP130;Pre mRNA splicing factor SF3b 130 kDa subunit;Pre-mRNA-splicing factor SF3b 130 kDa subunit;RSE1;SAP 130;SF3b130;SF3B3_HUMAN;Spliceosome associated protein 130;Splicing factor 3b subunit 3 130kD;STAF130

Research Areas:Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A553

UNIProt ID:Q15393

Accession:

Background:SF3B3 is one of four proteins for SF3B, which is involved in RNA splicing. Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occurred in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which composed of 4 proteins. SF3B3 is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence(BPS) in pre-mRNA.

Concentration:1 mg/mL

Immunogen:Recombinant human SF3B3 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:500-1:2000;IP 1:25

Calculated MW:135 kDa

ObservedMW:135 kDa