Recombinant SARS-CoV-2 S-trimer(D614G)(His Tag)

SKU: PKSV030388

Size: 500μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: 2019-nCoV S glycoprotein;2019-nCoV Spike glycoprotein;2019-nCov S protein;SARS-nCoV-2 Spike glycoprotein

Research Areas:

Conjugation:

Target Species: SARS-CoV-2

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P0DTC2

Accession: QHD43416.1

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Concentration:

Activity: Immobilized Recombinant 2019-nCoV S-trimer Protein (His Tag)(D614G)(Active)(Cat#PKSV030388) at 2μg/ml (100 μl/well) can bind Recombinant Human ACE-2 Protein (Fc Tag)(Active)(Cat#PKSR030492). The ED50 of PKSR030492 is 31.75 ng/ml.

Sequence: Cys15-Gln1208(D614G)

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 136.5 kDa

ObservedMW: 170-220 kDa