Recombinant SARS-CoV-2 Nucleocapsid Protein

SKU: PKSV030309

Size: 500μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: SARS-CoV-2 coronavirus NP Protein;SARS-CoV-2 novel coronavirus Nucleoprotein Protein;SARS-CoV-2 np Protein

Research Areas:

Conjugation:

Target Species: SARS-CoV-2

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P0DTC2

Accession: QHD43423.2

Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Concentration:

Activity: Immobilized 2019-nCoV NP Antibody(6G9)-Fc at 5μg/ml(100 μl/well) can bind Recombinant 2019-nCoV Nucleocapsid Protein (Mammalian)-His. The ED50 of 2019-nCoV Nucleocapsid Protein-His is 0.172 ug/ml.

Sequence: Met1-Ala419

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 46.4 kDa

ObservedMW: 50 kDa