Recombinant SARS-CoV-2 Guanine-N7_meth Protein (His Tag)

SKU: PKSR030473

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation: Guanine-N7_meth

Target Synonym: Guanine-N7 methyltransferase;SARS-CoV 2 ExoN;SARS-CoV 2 nsp14

Research Areas:

Conjugation:

Target Species: SARS-CoV-2

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID:

Accession: YP_009725309.1

Background: The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.

Concentration:

Activity: Not validated for activity

Sequence: Ala1-Gln527

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 2mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.9 kDa

ObservedMW: 60 kDa