Recombinant Rhesus Macaque IL-1 Beta protein

SKU: CD00040

Size:2 ug

Background:Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. The mature Rhesus macaque IL1β shares 96 % amino acid sequence identity with rhesus and 67 % - 78 % with canine, mouse and rat IL1β.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rhesus macaque Interleukin 1 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1.G4.1 cells is less than 1 pg/ml, corresponding to a specific activity of > 1. × 18 IU/mg.

Synonyms:IL-1β,IL-1beta,IL1B, Monkey

Endotoxin:Less than 1 EU/μg of rRhIL-1β as determined by LAL method.

Storage:-20ºC

Sequence:APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS

uniprot accession:P79182

Molecular weight:Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.