Recombinant Rat VEGF165 protein

SKU: CD00066

Size:2 ug

Background:Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGFs normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF165 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of mouse, 88 % with human and bovine, 89 % with porcine and canine, and 90 % with feline and equine VEGF, respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Vascular Endothelial Growth Factor165

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:VEGF165, Rat

Endotoxin:Less than 1 EU/μg of rRtVEGF165 as determined by LAL method.

Storage:-20ºC

Sequence:MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

uniprot accession:P16612

Molecular weight:Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris, 3 mM NaCl, pH 8.8.