Recombinant Rat MIP-1 Alpha/CCL3 protein

SKU: CD00087

Size:5 ug

Background:Chemokine (C-C motif) ligand 3 encoded by the CCL3 gene, also known as macrophage inflammatory protein-1α (MIP-1α) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL3 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL3 contains 69 amino acids and it shares 72 % and 91 % a.a. sequence identity with human and Mouse CCL3. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.

Research_area:Others

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Macrophage Inflammatory Protein-1 alpha/CCL3

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 1-1 ng/ml.

Synonyms:MIP-1α/CCL3, MIP-1a, Scya3Rat

Endotoxin:Less than 0.1 EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.

Storage:-20ºC

Sequence:APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA

uniprot accession:P5229

Molecular weight:Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 3 % Acetonitrile and .1 % TFA.