Recombinant Rat IL-6/Interleukin-6 Protein

SKU: PKSR030420

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-6/Interleukin-6

Target Synonym: IL6

Research Areas:

Conjugation:

Target Species: Rat

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: A6KJN3

Accession: NP_036721.1

Background: Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Concentration:

Activity: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml.

Sequence: Phe25-Thr211

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 21.9 kDa

ObservedMW: 22 kDa