Recombinant Rat IL-4 protein

SKU: CD00103

Size:5 ug

Background:Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. IL-4 can enhance both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rat IL-4 shares about 40 % ~ 45 % a.a. sequence identity with human, mouse, rhesus macaque IL-4 and they are species-specific in their activities.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-4

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using rat splenocytes is less than 1. ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:IL-4, Il4e12,IL4Rat

Endotoxin:Less than 1 EU/μg of rRtIL-4 as determined by LAL method.

Storage:-20ºC

Sequence:HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

uniprot accession: P296

Molecular weight:Approximately 14. kDa, a single non-glycosylated polypeptide chain containing 125 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose.