Recombinant Rat HB-EGF protein

SKU: CD00118

Size:10 ug

Background:Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. In addition, it shares about 73 % and 76 % a.a. sequence identity with Mouse and rat HB-EGF.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Heparin-binding EGF-like Growth Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:HB-EGF, Dtr, GFHB, Hbegf, Hegfl,Rat

Endotoxin:Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.

Storage:-20ºC

Sequence:DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL

uniprot accession:Q6175

Molecular weight:Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, 3 mM NaCl, pH 7.4, 5 % trehalose.