Recombinant Phospho-Smad2 (S255) Monoclonal Antibody

SKU: AN302094L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Phospho-Smad2 (S255)

Target Synonym:MADH;MADR;hMAD;SMAD;hSMAD;JV18;JV18-1;MADH2;MADR2;hMAD-2;hSMAD2;Smad2;Drosophila;homolog of;hSMAD family member 2;JV181;MAD;MAD homolog 2;MAD Related Protein 2;Mad-related protein 2;MGC22139;MGC34440;Mother against DPP homolog 2;Mothers against decapentaplegic;Mothers against decapentaplegic homolog 2;Mothers against DPP homolog 2;OTTHUMP00000163489;Sma and Mad related protein 2;Sma- and Mad-related protein 2 MAD;SMAD 2;SMAD family member 2

Research Areas:Signal Transduction;Epigenetics and Nuclear Signaling;Stem Cells;Cancer;Kits;Lysates;Other;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A818

UNIProt ID:Q15796

Accession:

Background:SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.

Concentration:1 mg/mL

Immunogen:Recombinant Smad2 (phospho S255) fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000

Calculated MW:52 kDa

ObservedMW:58 kDa