Recombinant Phospho-PDPK1 (Ser241) Monoclonal Antibody

SKU: AN301308L

Size::

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Acetyl/Phospho/Methyl Antibodies;Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:PDPK1

Target Synonym:PDK;PRO;3-phosphoinositide-dependent protein kinase;PDPK1;PDK1;PDPK2;PDPK2P;PRO0461;3-phosphoinositide-dependent protein kinase 1;3-phosphoinositide-dependent protein kinase 1;PDK1;PDPK2;PDPK2P;PRO0461;3 phosphoinositide dependent protein kinase 1;hPDK 1;hPDK1;MGC20087;MGC35290;OTTHUMP00000159109;OTTHUMP00000159110;OTTHUMP00000174525;PkB kinase;PkB kinase like gene 1;PkB like 1;Protein kinase

Research Areas:Signal Transduction

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse;Rat

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:7C9

UNIProt ID:O15530

Accession:

Background:ATP + a protein = ADP + a phosphoprotein.,Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.

Concentration:0.2 mg/mL

Immunogen:A synthetic peptide corresponding to residues around (Ser241) of Human Phospho-PDPK1

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A

Dilution:WB 1:2000-1:10000;

Calculated MW:63 kDa

ObservedMW:63 kDa