Recombinant Phospho-HSP27 (Ser78) Monoclonal Antibody

SKU: AN302087L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Phospho-HSP27 (Ser78)

Target Synonym:SRP;HSPB;HEL-S;HSP;HSPB1;CMT2F;HEL-S-102;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27;28 kDa heat shock protein;DKFZp586P1322;epididymis secretory protein Li 102;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;heat shock protein family B (small) member 1;Hsp 25;HSP 27;Hsp 28;Hsp B1;Stress responsive protein 27;Stress-responsive protein 27

Research Areas:Signal Transduction;Cancer;Cardiovascular

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IHC;IF;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A811

UNIProt ID:P04792

Accession:

Background:Hsp27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization.

Concentration:1 mg/mL

Immunogen:phosphorylated human HSP27 (Ser78) peptide

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:100-1:500;IF 1:50;IP 1:50-1:100

Calculated MW:23 kDa

ObservedMW:27 kDa