Recombinant Mycobacterium tuberculosis 6KDA early secretory antigenic target (esxA), partial

SKU: P9WNK7

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P9WNK7

Gene Names: esxA

Alternative Name(s): ESAT-6

Abbreviation: Recombinant Mycobacterium tuberculosis esxA protein, partial

Organism: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

Source: Yeast

Expression Region: 6-95aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: WNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA

MW: 11.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Not known. Elicits high level of IFN-gamma from mory effector cells during the first phase of a protective immune response.

Reference: Mutually dependent secretion of proteins required for mycobacterial virulence.Fortune S.M., Jaeger A., Sarracino D.A., Chase M.R., Sassetti C.M., Sherman D.R., Bloom B.R., Rubin E.J.Proc. Natl. Acad. Sci. U.S.A. 102: 10676-10681(2005)

Function: A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen