Recombinant Mouse S100a9 protein (His Tag)

SKU: PDEM100228

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:S100a9

Target Synonym:Leukocyte L1 complex heavy chain;60B8AG;CAGB;CFAG;CGLB;Calgranulin B;Calgranulin-B;Calprotectin L1H subunit;Cystic fibrosis antigen B;L1AG;LIAG;Leukocyte L1 complex heavy chain;MAC387;MIF;Migration inhibitor;Migration inhibitory factor related protein 14

Research Areas:

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:Immunogen

Fusion tag:N-His & C-His

UNIProt ID:P31725

Accession:P31725

Background:S100A9 is a calcium-and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra-and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.

Concentration:

Activity:Not validated for activity

Sequence:Ala2-Lys113

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:14.3 kDa

ObservedMW:15-18 kDa