Recombinant Mouse MIP-1 Gamma/CCL9/CCL10 protein

SKU: CD00296

Size:5 ug

Background:Mouse CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a Mouse macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Mouse CCL9/10 is 75 % amino acid identical to rat CCL9/10.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Macrophage Inflammatory Protein-1 gamma/CCL9/CCL10

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of .1-1 ng/ml.

Synonyms:MIP-1γ/CCL9/CCL1, MRP; Scy; Scya; CCF18; MRP-2; Scya9; Scya1,Mouse

Endotoxin:Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.

Storage:-20ºC

Sequence:QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q

uniprot accession:P5167

Molecular weight:Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 11 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.