Recombinant Mouse Mesothelin/MSLN Protein (His Tag)

SKU: PKSM041304

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Mesothelin;MSLN

Target Synonym: CAK1;MSLN ;Mes;Mesothelin;Mpf;Msln

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q61468

Accession: Q61468

Background: Mesothelin is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF).The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is over expressed in several human tumors, including mesothelioma and ovarian and pancreatic adenocarcinoma. The interaction between mesothelin and MUC16 (also known as CA125) may facilitate the implantation and peritoneal spread of tumors by cell adhesion. The region (296-359) consisting of 64 amino acids at the N-terminal of cell surface mesothelin is the functional binding domain for MUC16. Mesothelin is a tumour differentiation antigen that is normally present on the mesothelial cells lining the pleura, peritoneum and pericardium. A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages.

Concentration:

Activity: Not validated for activity

Sequence: Asp298-Ser600

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 35.0 kDa

ObservedMW: 38-55 kDa