Recombinant Mouse M-CSF protein

SKU: CD00305

Size:2 ug

Background:Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229 a.a. of mature mouse M-CSF shares 87 %, 83 %, 82 % and 81 % sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Macrophage Colony Stimulating Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse M-NFS-6 cells is less than 2 ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:M-CSF,CSF1,op; Csf; CSF-; Csfm; M-CS; MCSF; C87615, Mouse

Endotoxin:Less than 1 EU/μg of rMuM-CSF as determined by LAL method.

Storage:-20ºC

Sequence:KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE

uniprot accession:P7141

Molecular weight:Approximately 52. kDa, a disulfide-linked homodimer consisting of two 23 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in 2 mM Tris, 5 mM NaCl, pH 7.4.