Recombinant Mouse IL-7 protein

SKU: CD00351

Size:2 ug

Background:Interleukin-7 (IL-7) is encoded by the IL7 gene in mouse and secreted by stromal cells in the red marrow and thymus. The protein signals through the IL-7 receptor, which is a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and it can stimulate proliferation of B cells, T cells and NK cells. Mouse IL-7 has approximately 65 % and 88 % amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Interleukin-7; Mouse IL-7

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse 2E8 cells is less than .2 ng/ml, corresponding to a specific activity of > 5. × 16 IU/mg.

Synonyms:IL-7, A6326I6Rik, Il, Il7, hlb36, hlb368Mouse

Endotoxin:Less than 1 EU/μg of rMuIL-7 as determined by LAL method.

Storage:-20ºC

Sequence:ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI

uniprot accession:P1168

Molecular weight:Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.