Recombinant Mouse IL-27 EBI3 protein(His Tag)

SKU: PKSM041472

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-27 EBI3

Target Synonym: EBI;Lymphopoietin 1(LP-1);pre-B-cell factor;Il27b;Ebi3

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: O35228

Accession: O35228

Background: Possesses antiviral functions and induces the type I intereferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1 (By similarity).

Concentration:

Activity: Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus.The ED₅₀ for this effect is <5 ng/mL.

Sequence: Ala 23-Pro 228

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 23.8 kDa

ObservedMW: 25 kDa