Recombinant Mouse ICOS Ligand/ICOSL Protein (His Tag)

SKU: PKSM041331

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: ICOS Ligand;ICOSL

Target Synonym: B7 homolog 2;B7-H2;B7-like protein Gl50;B7H2;B7RP-1;B7RP-1LICOS;B7RP1;CD275;CD275 antigen;GL50;ICOS Ligand;ICOS ligand;ICOS-L;ICOSL;ICOSLG;inducible T-cell co-stimulator ligand

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q9JHJ8

Accession: Q9JHJ8

Background: Mouse ICOS ligand(B7-H2) is an approximately transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation. The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells and germinal centers. B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung. Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs antitumor immunity. In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones.

Concentration:

Activity: Not validated for activity

Sequence: Glu47-Lys279

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.1 kDa

ObservedMW: 40-60 kDa